gDNA Target Sequencing
Sanger Sequencing for gDNA targets (from 300 bps to 1.5 kb).
Genomic DNA target sequencing is designed to detect sequence variants in short genomic DNA regions. The target sequence is amplified by PCR using purified gDNA and customer-provided primers or ACGT-designed primers, producing an amplicon which can be sequenced by Sanger. The sequence data and variant analysis results are publication-quality.
Service Description
- QC check of genomic DNA samples
- Amplification of target regions using customer-supplied amplification primers or synthesized primers
- Performance of Sanger sequencing reactions using same amplification primers, customer-supplied sequencing primers, or synthesized primers
- Internal sequencing primer design and synthesis if the target region is longer than 600 bps
- Sequencing reaction cleanup with magnetic beads for superior quality traces
- Analysis of the extension products using our ABI 3730 Genetic Analyzers
- Data review and editing of chromatograms from each sample
- Consensus sequence generation and variant analysis (if reference sequence is provided)
Highlights
- Generates 750 to 1,000 bases of data
- Extensive troubleshooting for unsuccessful samples
- Repeat reactions provided at no additional cost
- Free primer banking and template storage by request
- Free use of ACGT universal primers
- Consensus sequence and variant analysis provided
- Provides Phred 20 quality sequences
- Free shipping via ACGT drop boxes
- Real-time project tracking
- 24/7 online ordering and data retrieval
- Dedicated technical support staff
How to Order
In most cases, sequencing data and analysis results are delivered within 2 weeks of sample receipt.
Are you looking to sequence longer targets, up to Whole Genome Sequencing (WGS)?
ACGT offers Next Generation Sequencing (NGS) technologies with an unparalleled throughput, scalability and speed. Both short-read and long-read capabilities are available via Illumina and Oxford Nanopore Technologies (ONT).
Are you looking to sequence longer targets, up to Whole Genome Sequencing (WGS)?
ACGT offers Next Generation Sequencing (NGS) technologies with unparalleled throughput, scalability and speed. Both short-read and long-read capabilities are available via Illumina and Oxford Nanopore Technologies (ONT).