Full Sequence Analysis
Sanger sequencing of large plasmids or PCR products (over 1.5 kb in length) using the primer walking method. Single or double stranded coverage available.
This service can be used to confirm the sequences of a longer area of interest or to detect mutations or deletions in a sequence. Primers may be customer-provided or designed by ACGT using a reference sequence to cover the length of the sequence. The sequence data and variant analysis results are publication-quality.
Single-Stranded Coverage
- 1x coverage of a stretch of DNA using the primer walking method.
- Primer Design and Synthesis is available if needed to cover the length of the sequence. Specific internal primers can be designed every 600-700 bps using a reference sequence or based on the results of a single reaction for primer walking along the length of the sequence. Primers can be banked at our facility for future use.
- Analysis of reactions using our ABI 3730 genetic analyzer.
- Thorough examination of chromatograms and data editing for quality assurance.
- Alignment of sequences to determine a single consensus sequence with the overlapping regions.
- Verification of each base pair during alignment to check for discrepancies.
- Variant analysis report in comparison with reference sequence.
Double-Stranded Coverage
- 2x coverage of a stretch of DNA using the primer walking method.
- Primer Design and Synthesis is available if needed to cover the length of the sequence. Specific internal primers can be designed every 600-700 bps using a reference sequence or based on the results of a single reaction for primer walking along the length of the sequence. Primers can be banked at our facility for future use.
- Analysis of reactions using our ABI 3730 genetic analyzer.
- Thorough examination of chromatograms and data editing for quality assurance.
- Alignment of sequences to determine a single consensus sequence with the overlapping regions.
- Verification of each base pair during alignment to check for discrepancies.
- Variant analysis report in comparison with reference sequence.
Are you interested in sequencing whole plasmids, BACs, or whole genomes with innovative sequencing technologies?
ACGT offers Next Generation Sequencing (NGS) technologies with unparalleled throughput, scalability and speed. Both short-read and long-read capabilities are available via Illumina and Oxford Nanopore Technologies (ONT).
