Plasmids & Long Amplicons
Full-length sequencing of purified plasmid DNA or long PCR products.
Full-length deep sequencing of purified plasmid DNA (up to 100kb) or long PCR products (from 0.5kb to 50kb), using state-of-the-art Illumina® NGS platform, with bioinformatics analysis included.
Service Description
- Construction of an NGS library from DNA sample, or from plasmid amplified from culture sample, with all required QC steps
- Sequencing of the library with Illumina short reads, generating comprehensive coverage with high depth and accuracy
- Demultiplexing, trimming, and quality-filtering of raw sequencing reads
- Sequence alignment of reads to provided reference sequence, or de novo assembly into larger contigs
Bioinformatics Analysis
Standard types of bioinformatics analysis are included with NGS services.
Map to Reference
Reads are aligned to the provided reference sequence to detect genetic variations (SNVs, InDels, CNVs) and generate a consensus sequence.
De Novo Assembly
Reads are self-assembled into a larger, scaffolded set of contigs, representative of the original sequence, without any reference.
Custom Analysis
Complex bioinformatics analysis for various research purposes can be arranged as a custom project.
Acceptable Target Types
- Episomes (up to 100 kb in length)
- Plasmids
- Cosmids
- Phagemids
- BACs
- PCR Products (0.5 kb to 50 kb in length)
Purified DNA Requirements
Please provide at least 10 µL, with OD260/280 >1.6 and <2.2, and all samples normalized to 3 ng/µL.
ACGT recommends DNA quantification using fluorometry. Quantification using Nanodrop is discouraged. Data quality of samples failing specified quantity and quality requirements cannot be guaranteed.
Bacterial Colony
Please provide sample colonies on agar plates, with 1 plate/sample.
Bacterial Culture
Please provide sample culture in tubes or plates, with at least 0.2 mL per sample.
For bacterial colony and bacterial culture samples, additional $50 per sample applies for plasmid template amplification.
Highlights
- High quality NGS reads at Q30 or higher (>99.9% accuracy)
- > 500X average depth over entire target sequence
- 2-week turnaround time in most cases
- Detect base deviations and small indels at higher sensitivity
- Cost-effective for medium to large targets longer than 2 kb
- Standard bioinformatics analysis included at no additional cost
- 24/7 online ordering and data retrieval
- Dedicated technical support staff
- Free overnight shipping via ACGT drop boxes
- Real-time project tracking
Submitting Your Samples
For detailed sample submission requirements, download the guide below.
