Next Generation Sequencing (OLD)
Committed to providing comprehensive NGS solutions.
ACGT is provides comprehensive solutions, from standard to custom services, including extraction, flexible library prep options, short-read and long-read sequencing and robust bioinformatics capabilities.
Committed to Quality
Our NGS services are offered at the following quality grades, up to cGMP compliance. ACGT pursues a culture of quality with an aim towards continuous improvement. Our rigorous quality management systems (QMS) ensure compliance with Good Laboratory Practices (GLP) and current Good Manufacturing Practices (cGMP) sequence analysis in regulatory grade sequencing and molecular biology studies.
Services
Short-read Sequencing
ACGT is a certified service provider for Illumina, the leading NGS platform. We offer advanced and highly reliable Illumina short-read technology for a variety of both DNA and RNA analysis applications:
- Target Sequence Characterization, from PCR products to full genomes
- Viral Genome and Viral Vector sequence analysis (both DNA and RNA viruses and phages)
- Detection of DNA and RNA pathogens in a variety of environmental and physiological samples
- Integration Site analysis of transgenic sequences in Master Cell Banks
- Microbiome profiling based on either short universal barcodes or on shotgun sequencing and de novo assembly
- Epigenetic profiling of DNA modifications
- Comprehensive gene expression analysis and transcriptomic profiling, offering valuable insights into gene expression patterns in both eukaryotes and prokaryotes, alternative splicing, and non-coding RNA
- Short RNA analysis (both discovery and differential expression) for miRNA and siRNA.
Long-read Sequencing
ACGT offers Oxford Nanopore Technologies (ONT) long-read sequencing. Benefits of ONT sequencing include its generation of exceptionally long sequencing reads, direct RNA sequencing, and real-time “organic” detection of base modifications on both DNA and RNA molecules. Long-read NGS technology provides several key advantages in the following workflows:
- Improved de novo genome assembly
- Enhanced structural variant detection
- Accurate haplotype phasing
- Comprehensive transcriptome analysis of full-length RNA molecules, allowing direct isoform detection
- Direct epigenetic profiling of both DNA and RNA
- Direct metagenomic resolution
- In-depth characterization of repeat regions.
Long vs Short NGS Reads
Which type of analysis is best for your project?
Short-Read Analysis
Repeat regions may cause gaps in the genome coverage with short reads, and loss of detection of some variants. But high complexity regions have high coverage levels and very high sequence accuracy
Coverage of high-complexity regions
Very high
Coverage of low-complexity regions
Low to None
Sequence quality/accuracy
Very high
Large Structural Variant analysis
Poor
Allele Phasing Analysis
Poor
Cost per Gb of data
Low
Long-Read Analysis
Long reads span large repeat regions and other structural variants for a full target coverage; however, coverage levels are usually lower, and sequence accuracy is not as good
Coverage of high-complexity regions
Low to Moderate
Coverage of low-complexity regions
Low to Moderate
Sequence quality/accuracy
Moderate
Large Structural Variant analysis
Good
Allele Phasing Analysis
Good
Cost per Gb of data
Moderate to High
Related Services
To complement our NGS services, ACGT offers the following:
SNP Genotyping
Copy Number Variation
Sanger Sequencing
Bioinformatics
NGS High-Throughput
- Starting at $120 per sample for plasmids and PCR products
- Starting at $200 per sample for bacterial WGS
- 500X average target coverage at >99.9% base call accuracy
- 2-week turnaround time
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