Next Generation Sequencing

Most advanced and proven NGS technologies for your sequencing needs.

ACGT is committed to using the most proven and up-to-date technologies in our Next Generation Sequencing (NGS) services, providing high coverage depth and accuracy, with low cost and fast turnaround.

Services

ACGT offers a number of NGS service options that are tailored to meet the needs of your projects, ranging from single-tube samples to multiple plate processing and data assembly

Committed to Quality

Our NGS services are offered at the following quality grades, up to cGMP compliance. ACGT pursues a culture of quality with an aim towards continuous improvement. Our rigorous quality management systems (QMS) ensure compliance with Good Laboratory Practices (GLP) and current Good Manufacturing Practices (cGMP) sequence analysis in regulatory grade sequencing and molecular biology studies.

Bioinformatics Analysis

Standard types of bioinformatics analysis are included with NGS services.

Map to Reference

Reads are aligned to the provided reference sequence to detect genetic variations (SNVs, InDels, CNVs) and generate a consensus sequence.

De Novo Assembly

Reads are self-assembled into a larger, scaffolded set of contigs, representative of the original sequence, without any reference.

Metagenomics

Reads are mapped to public databases and assigned microorganism species identity and relative abundance in the sample.

Custom Analysis

Complex bioinformatics analysis for various research purposes can be arranged as a custom project.

Your data matters.

ACGT ensures your data belongs only to you.

ACGT is a 100% U.S.-based company. All generated data is managed and stored onsite, at our headquarter and labs, by dedicated IT and bioinformatics teams.

Deliverable data is encrypted, archived, and purged for security, integrity, and confidentiality.

Services

Short-read Sequencing

ACGT is a certified service provider for Illumina, the leading NGS platform. We offer advanced and highly reliable Illumina short-read technology for a variety of both DNA and RNA analysis applications:

Target Sequence Characterization, from PCR products to full genomes
Viral Genome and Viral Vector sequence analysis (both DNA and RNA viruses and phages)
Detection of DNA and RNA pathogens in a variety of environmental and physiological samples
Integration Site analysis of transgenic sequences in Master Cell Banks
Microbiome profiling based on either short universal barcodes or on shotgun sequencing and de novo assembly
Epigenetic profiling of DNA modifications
Comprehensive gene expression analysis and transcriptomic profiling, offering valuable insights into gene expression patterns in both eukaryotes and prokaryotes, alternative splicing, and non-coding RNA
Short RNA analysis (both discovery and differential expression) for miRNA and siRNA.

Long-read Sequencing

ACGT offers Oxford Nanopore Technologies (ONT) long-read sequencing. Benefits of ONT sequencing include its generation of exceptionally long sequencing reads, direct RNA sequencing, and real-time “organic” detection of base modifications on both DNA and RNA molecules. Long-read NGS technology provides several key advantages in the following workflows:

Improved de novo genome assembly
Enhanced structural variant detection
Accurate haplotype phasing
Comprehensive transcriptome analysis of full-length RNA molecules, allowing direct isoform detection
Direct epigenetic profiling of both DNA and RNA
Direct metagenomic resolution
In-depth characterization of repeat regions.

Compare Services

Choose the sequencing service most suitable for your project.

Sanger Sequencing

Conventional Sanger Sequencing is quick, reliable, and high-quality. It is limited by its scale and throughput for full genome coverage and bioinformatic analysis.

General Target Coverage

Low

Coverage of low-complexity regions

Low

Sequence quality/accuracy

High

Large Structural Variant analysis

Moderate

Allele Phasing Analysis

Poor

Cost per Gb of data

Very High

Short-Read Sequencing

Target regions have very high coverage levels and sequence accuracy with short reads. Repeat regions may cause gaps in the genome coverage and loss of large variant detection.

General Target Coverage

Very high