Transgenic Sequence Insertion Site Analysis
Rapid, accurate mapping of integration site of transgenic sequences in Master Cell Banks.
ACGT’s transgenic sequence characterization assays capture the rapid development and high throughput of NGS sequencing platforms, coupled with powerful bioinformatic tools, generating an insightful combination of whole-genome sequencing (WGS) and sequence alignment/de novo assembly that reliably characterizes copy number, insertion loci, and flanking nucleotides in transgenic sequences in Master Cell Banks or modified cell lines. This approach can also be used to determine insertion sites of retroviral or lentiviral genomes.
NGS sequenced reads are mapped, classified, and/or assembled to characterize the integration site by sequence alignment or de novo assembly.
Committed to Quality
Our NGS services are offered at the following quality grades, up to cGMP compliance. ACGT pursues a culture of quality with an aim towards continuous improvement. Our rigorous quality management systems (QMS) ensure compliance with Good Laboratory Practices (GLP) and current Good Manufacturing Practices (cGMP) sequence analysis in regulatory grade sequencing and molecular biology studies.
