NEXT GENERATION SEQUENCING
ACGT is committed to using the most proven and up-to-date technologies in our Next Generation Sequencing (NGS) service. We provide SBS (sequencing by synthesis) TruSeq technology from Illumina®/Solexa, the most successful NGS platform adopted worldwide. TruSeq’s dependable base-by-base sequencing delivers the industry’s most accurate data for a broad range of applications. Furthermore, ACGT uses only data that has met or exceed the Q30 (i.e. 1 out of 1,000 chance of error) threshold in our analyses.
Platforms and Capabilities
Our Illumina MiSeq®, HiSeq4000® and NextSeq500® instruments are established and versatile platforms for genomic discovery and validation.
Illumina MiSeq®
| Capacity: | Single lane flow cell |
| Maximum Clusters: | 20 to 25 million (up to 50 million paired-end reads) |
| *Maximum Run: | 2 x 300 bp paired-end, 65 hours, up to 12 Gb of sequence data |
| Expected PE300 Read Quality: | > 70% Q30 bases per run |
Illumina NextSeq500®
| Capacity: | Mid Output Mode Run | High-Output Run |
| *Maximum Clusters: | 120 million PE reads | 400 million PE reads |
| *Maximum Run: | 2 x 150 bp paired-end, 26 hours, up to 36 Gb of sequence data | 2 x 150 bp paired-end, 29 hours, up to 110 Gb of sequence data |
| Expected PE150 Read Quality: | > 75% Q30 bases per run | > 75% Q30 bases per run |
Illumina HiSeq4000®
| Capacity: | One 8-lane flow cell | Two 8-lane flow cells |
| *Maximum Clusters: | 2.5 billion PE reads | 5 billion PE reads |
| *Maximum Run: | 2 x 150 bp paired-end, 3.5 days, up to 750 Gb of sequence data | 2 x 150 bp paired-end, 3.5 days, up to 1,500 Gb of sequence data |
| Expected PE150 Read Quality: | > 75% Q30 bases per run | > 75% Q30 bases per run |
# While a total of 384 samples can be uniquely barcoded with Nextera Illumina indexes, 1 barcode may be reserved for the PhiX control on all lanes.
*Based on the v3 Illumina kits
Next Generation Sequencing Applications We Support
- Whole Genome and Whole Exome Sequencing Analysis
- Targeted Sequence Analysis
- Transcriptome (RNA-Seq) and small RNA Analysis
- DNA Methylation Analysis
- ChIP-Seq Analysis
- Metagenomic Analysis
The following are included with our NGS services:
- Extensive project consultation to help you optimize your experiment
- Complete Quality Control analysis during library preparation
- Onsite sample processing and custom library preparation
- Onsite RT-qPCR analysis available for evaluation of key transcript levels in sample RNA
- Data processing and bioinformatics analysis can be performed entirely onsite if requested, for maximum data security and confidentiality
- High quality raw sequence data (provided as FASTA files) and all bioinformatics analysis files are shipped on a drive or provided through a secure FTP link
- Multiple data analysis options are available
- Full project report
- Sanger sequencing is available onsite for gap closure, SNP validation and junction sequence confirmation
NGS Service FAQs
Dr. Semyon Rubinchik, Scientific Director, answers frequently asked questions about our NGS services.
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