SAMPLE SUBMISSION GUIDELINES

Samples can be submitted in two different formats: raw material (tissue, cells, soil, etc.), or purified nucleic acids (DNA or RNA). Please read carefully the description of the quantity and quality requirements for each type of sample.

A failure to provide the required amounts and quality of sample material may downgrade the performance of service(s) and/or make the completion of the order unattainable.

DNA/RNA Extraction Services

 

Details of Service:

1.  All extracted DNA samples will be evaluated for quantity and quality with NanoDrop spectroscopy and/or Qubit fluorometry and/or agarose gel electrophoresis, as appropriate.

2.  All extracted RNA samples will be evaluated for quantity and quality (RIN values) with NanoDrop spectroscopy and/or Qubit fluorometry, and 2100 Bioanalzyer with RNA 6000 chip.

3.  Any significant issues with sample quantity or quality shall be communicated to the individual requesting the service.

 

DNA/RNA Extraction Services Amount of Required Material*
DNA or RNA extraction -standard (blood, soft tissue, swabs, saliva, cells, bacteria and yeasts) 0.5 mL of blood; 20 mg of tissue, 2 swabs, 1 mL of saliva, 106 cells, 109 cells or several colonies on a plate
DNA extraction -difficult (FFPE, bone, biofilm, soil, fungi spores, plants) 5 slides, 100 mg of tissue, 50 mg of biofilm, 1 gm of soil, 100 mg of fungal material, 200 mg of plant tissue
RNA extraction – standard 0.5 mL of blood; 20 mg of tissue, 2 swabs, 1 mL of saliva, 106 cells
RNA extraction – difficult 100 mg of tissue; 50 mg of biofilm, 1 gm of soil, 100 mg of fungal material, 200 mg of plant tissue
RNA extraction FFPE 6 slides or equivalent

*The amounts identified above are the minimum requirements. We strongly recommend that your submission exceed these standards, if possible.

DNA / RNA samples

Type of Material Amount of Material Sample Quality Requirements
Genomic DNA 100 ng OD260/280 between 1.6 and 2.0;OD260/230 more than 1.6; concentration between 20 and 500 ng / µL
DNA amplicons 50 ng/µl per band as determined by 2100 Bioanalyzer or Fragment Analyzer
Total RNA 2,000 ng OD260/280 more than 2.0;OD260/230 more than 1.8; concentration between 20 and 500 ng / µL; RIN>8.0
mRNA 500 ng