Request a quote for further details. To place an order, please log in to your account and enter your order on the High Throughput online order form.
ACGT offers high throughput DNA sequencing in a fully automated lab. Our DNA sequencing team is capable of handling thousands of clones per day using a barcode system to accurately inventory and process DNA samples upon receipt until delivery of final data. Project tracking for each stage of sample processing can be viewed in real-time online via our website.
High Throughput DNA Sequencing Service:
Samples can be submitted as extracted DNA samples or as colonies or culture. We offer the following service options:
- Premium– A few samples and primers will be randomly quantified using agarose gel and nanodrop. The concentration values will then be generalized for the whole plate. The concentration of all the samples and primers submitted should be comparable throughout the plate. Unpurified PCR products will be purified before sequencing.
- Standard– Provide us with the sample and primer concentration and we will optimize the sequencing reactions accordingly. The concentration value provided should be representative for the whole plate
- LCO– This is the most cost effective service. Send us the template and primer premixed according to our sample submission guidelines. Universal primers will be provided at no extra charge
For our sample submission requirements, please click here.
Service description:
- Checking template and primer DNA using Nanodrop or gel electrophoresis
- Cycle sequencing reactions using BigDye terminator version 3.1 from ABI
- Clean-up using CleanSEQ dye terminator removal kit on Biomek Automation Station from Beckman Coulter, Inc.
- Analysis of the extension products using ABI 3730XL
- A thorough examination of the chromatograms and data editing
- Data review of each sample and return of review summary
- Return of 600-900 bases of edited and vector clipped sequences to optimize the database searches
- Universal primers provided at no additional charge
Related Services
“Load & Go” Service:
With this service, you perform your own DNA sequencing reactions with BigDye® Terminator v3.1 enzyme and send us the cleaned or uncleaned samples for sequencing. Samples can be submitted either in liquid or dried format. Select the Low Cost Option on the order form and mention in the comments section that the service is “Load & Go”
Service Description:
- Clean up of uncleaned sequencing reactions with CleanSEQ® magnetic beads.
- Analysis of your reactions on an ABI 3730XL or 3730
- A thorough examination of the chromatograms and data editing
- Data review of each DNA sequencing sample and return of review summary
- Next-day data return for standard service
- “Same Day Service” available