DATA ANALYSIS & RETRIEVAL
SAMPLE SUBMISSION REQUIREMENTS
FAQs

FULL SEQUENCING ANALYSIS (FSA)

Full DNA Sequencing AnalysisACGT offers a Full Sequence Analysis (FSA) service to confirm the sequences of a clone or a detection of mutations or deletions. The results from our comprehensive FSA service are publication quality. Pricing for this service is project specific. Please click here to request for a quote for your project.

The FSA service is appropriate for regions or samples of interest greater than 2 Kb in length. For samples less than 2 Kb, please use our Single Pass Primer Extension Services.

Double-Stranded Full Sequence Analysis Service Description:
  • DNA sequencing by the primer walking method with multiple overlap
  • Design and synthesis of internal primers specific to your template DNA at every 600-700 bases. Primers will be banked at ACGT for future orders
  • Thorough examination of the chromatograms and data editing
  • Sequence data assembly and alignment
  • Check and verify every base for discrepancies
  • Determination of consensus sequence
  • Variant analysis report in comparison with reference sequence
Single-Stranded Full Sequence Analysis Service Description:FSA2
  • Design and synthesis of internal primers specific to your template DNA at every 600-650 bases in single strand. Primers will be banked at ACGT for future orders.
  • Thorough examination of the chromatograms and data editing
  • Overlapping sequence data assembly and alignment
  • Check and verify every base for discrepancies in overlapping
    regions
  • Determination of consensus sequence in overlapping regions
  • Variant analysis report in comparison with reference sequence
REQUEST A QUOTE